SPELL - Nematode

Citation	Friday AJ, Henderson MA, Morrison JK, Hoffman JL, Keiper BD. Spatial and temporal translational control of germ cell mRNAs via an eIF4E isoform, IFE-1. J Cell Sci, 2015.
PubMed ID	26542024
Short Description	Spatial and temporal translational control of germ cell mRNAs via an eIF4E isoform, IFE-1. GEO Record: GSE74459 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00048771.ce.mr.csv
# of Conditions	12
Full Description	Regulated mRNA translation is vital for germ cells to produce new proteins in the spatial and temporal patterns that drive gamete development. Translational control involves the de-repression of stored mRNAs and their recruitment by initiation factors (eIF's) to ribosomes. C. elegans expresses five eIF4Es (IFE-1-5); several were shown to selectively recruit unique pools of mRNA. Individual IFE knockouts yield unique phenotypes due to inefficient translation of certain mRNAs. We identified mRNAs preferentially translated via a germline-specific eIF4E isoform, IFE-1. Differential polysome microarray analysis identified 77 mRNAs recruited by IFE-1. Among the IFE-1-dependent mRNAs are several required for late germ cell differentiation and maturation. Polysome association of gld-1, vab-1, vpr-1, rab-7, and rnp-3 mRNAs relies on IFE-1. Live animal imaging showed IFE-1-dependent selectivity in spatial and temporal translation of germline mRNAs. Altered MAPK activation in oocytes suggests dual roles for IFE-1, both promoting and suppressing oocyte maturation at different stages. This single eIF4E isoform exerts positive, selective translational control during germ cell differentiation. Experimental Details: WBPaper00048771:non-polysomal_ife-1(bn127)_rep1 WBPaper00048771:polysomal_ife-1(bn127)_rep1 WBPaper00048771:non-polysomal_N2_rep1 WBPaper00048771:polysomal_N2_rep1 WBPaper00048771:non-polysomal_ife-1(bn127)_rep2 WBPaper00048771:polysomal_ife-1(bn127)_rep2 WBPaper00048771:non-polysomal_N2_rep2 WBPaper00048771:polysomal_N2_rep2 WBPaper00048771:non-polysomal_ife-1(bn127)_rep3 WBPaper00048771:polysomal_ife-1(bn127)_rep3 WBPaper00048771:non-polysomal_N2_rep3 WBPaper00048771:polysomal_N2_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: WT vs. mutant