| Citation | McJunkin K, Ambros V. A microRNA family exerts maternal control on sex determination in C. elegans. Genes Dev, 2017. |
| PubMed ID | 28279983 |
| Short Description | A microRNA family exerts maternal control on sex determination in C. elegans. GEO Record: GSE94701 GSE94702 GSE94704 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00050903.ce.mr.csv |
| # of Conditions | 12 |
Full Description
|
Gene expression in early animal embryogenesis is in large part controlled post-transcriptionally. Maternally contributed microRNAs may therefore play important roles in early development. We elucidated a major biological role of the nematode mir-35 family of maternally contributed essential microRNAs. We show that this microRNA family regulates the sex determination pathway at multiple levels, acting both upstream of and downstream from her-1 to prevent aberrantly activated male developmental programs in hermaphrodite embryos. Both of the predicted target genes that act downstream from the mir-35 family in this process, suppressor-26 (sup-26) and NHL (NCL-1, HT2A, and LIN-41 repeat) domain-containing-2 (nhl-2), encode RNA-binding proteins, thus delineating a previously unknown post-transcriptional regulatory subnetwork within the well-studied sex determination pathway of Caenorhabditis elegans Repression of nhl-2 by the mir-35 family is required for not only proper sex determination but also viability, showing that a single microRNA target site can be essential. Since sex determination in C. elegans requires zygotic gene expression to read the sex chromosome karyotype, early embryos must remain gender-naive; our findings show that the mir-35 family microRNAs act in the early embryo to function as a developmental timer that preserves naivete and prevents premature deleterious developmental decisions. Experimental Details: WBPaper00050903:mir-35-41(nDf50)_20C_rep1 WBPaper00050903:mir-35-41(nDf50)_20C_rep2 WBPaper00050903:mir-35-41(nDf50)_20C_rep3 WBPaper00050903:N2_20C_rep1 WBPaper00050903:N2_20C_rep2 WBPaper00050903:N2_20C_rep3 WBPaper00050903:mir-35-41(nDf50)_25C_rep1 WBPaper00050903:mir-35-41(nDf50)_25C_rep2 WBPaper00050903:mir-35-41(nDf50)_25C_rep3 WBPaper00050903:N2_25C_rep1 WBPaper00050903:N2_25C_rep2 WBPaper00050903:N2_25C_rep3. |
|
Tags
|
Contact: help (at) wormbase.org
