SPELL - Nematode

Citation	Hou L, Wang D, Chen D, Liu Y, Zhang Y, Cheng H, Xu C, Sun N, McDermott J, Mair WB, Han JD. A Systems Approach to Reverse Engineer Lifespan Extension by Dietary Restriction. Cell Metab, 2016.
PubMed ID	26959186
Short Description	A Systems Approach to Reverse Engineer Lifespan Extension by Dietary Restriction. GEO Record: GSE77109 GSE77110 GSE77111 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00049364.ce.mr.csv
# of Conditions	35
Full Description	Dietary restriction (DR) is the most powerful natural means to extend lifespan. Although several genes can mediate responses to alternate DR regimens, no single genetic intervention has recapitulated the full effects of DR, and no unified system is known for different DR regimens. Here we obtain temporally resolved transcriptomes during calorie restriction and intermittent fasting in Caenorhabditis elegans and find that early and late responses involve metabolism and cell cycle/DNA damage, respectively. We uncover three network modules of DR regulators by their target specificity. By genetic manipulations of nodes representing discrete modules, we induce transcriptomes that progressively resemble DR as multiple nodes are perturbed. Targeting all three nodes simultaneously results in extremely long-lived animals that are refractory to DR. These results and dynamic simulations demonstrate that extensive feedback controls among regulators may be leveraged to drive the regulatory circuitry to a younger steady state, recapitulating the full effect of DR. Experimental Details: WBPaper00049364:aak-2;daf-2(e1370)rsks-1(ok1255)_control_rep1 WBPaper00049364:aak-2;daf-2(e1370)rsks-1(ok1255)_control_rep2 WBPaper00049364:aak-2_control_rep1 WBPaper00049364:aak-2_control_rep2 WBPaper00049364:daf-2(e1370)_control_rep1 WBPaper00049364:daf-2(e1370)_control_rep2 WBPaper00049364:daf-2(e1370)_tax-6(RNAi)_rep1 WBPaper00049364:daf-2(e1370)_tax-6(RNAi)_rep2 WBPaper00049364:daf-2(e1370)rsks-1(ok1255)_control_rep1 WBPaper00049364:daf-2(e1370)rsks-1(ok1255)_control_rep2 WBPaper00049364:daf-2(e1370)rsks-1(ok1255)_tax-6(RNAi)_rep1 WBPaper00049364:daf-2(e1370)rsks-1(ok1255)_tax-6(RNAi)_rep2 WBPaper00049364:N2_control_rep1 WBPaper00049364:N2_control_rep2 WBPaper00049364:N2_tax-6(RNAi)_rep1 WBPaper00049364:N2_tax-6(RNAi)_rep2 WBPaper00049364:rsks-1(ok1255)_control_rep1 WBPaper00049364:rsks-1(ok1255)_control_rep2 WBPaper00049364:rsks-1(ok1255)_tax-6(RNAi)_rep1 WBPaper00049364:rsks-1(ok1255)_tax-6(RNAi)_rep2 WBPaper00049364:N2_free-feeding_AdultDay2 WBPaper00049364:N2_free-feeding_AdultDay4 WBPaper00049364:N2_calorie-restriction_AdultDay4 WBPaper00049364:N2_intermittent-fasting_AdultDay4 WBPaper00049364:N2_free-feeding_AdultDay6 WBPaper00049364:N2_calorie-restriction_AdultDay6 WBPaper00049364:N2_intermittent-fasting_AdultDay6 WBPaper00049364:N2_free-feeding_AdultDay8 WBPaper00049364:N2_calorie-restriction_AdultDay8 WBPaper00049364:N2_intermittent-fasting_AdultDay8 WBPaper00049364:N2_free-feeding_AdultDay10 WBPaper00049364:N2_calorie-restriction_AdultDay10 WBPaper00049364:N2_intermittent-fasting_AdultDay10 WBPaper00049364:N2_intermittent-fasting_AdultDay12 WBPaper00049364:N2_intermittent-fasting_AdultDay14.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: response to starvation