SPELL - Nematode

Citation	Noble D, Aoki ST, Ortiz MA, Kim KW, Verheyden JM, Kimble J. Genomic Analyses of Sperm Fate Regulator Targets Reveal a Common Set of Oogenic mRNAs in Caenorhabditis elegans. Genetics, 2016.
PubMed ID	26564160
Short Description	Genomic Analyses of Sperm Fate Regulator Targets Reveal a Common Set of Oogenic mRNAs in Caenorhabditis elegans. GEO Record: GSE73070 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00048762.ce.mr.csv
# of Conditions	40
Full Description	Germ cell specification as sperm or oocyte is an ancient cell fate decision, but its molecular regulation is poorly understood. In Caenorhabditis elegans, the FOG-1 and FOG-3 proteins behave genetically as terminal regulators of sperm fate specification. Both are homologous to well-established RNA regulators, suggesting that FOG-1 and FOG-3 specify the sperm fate post-transcriptionally. We predicted that FOG-1 and FOG-3, as terminal regulators of the sperm fate, might regulate a battery of gamete-specific differentiation genes. Here we test that prediction by exploring on a genomic scale the messenger RNAs (mRNAs) associated with FOG-1 and FOG-3. Immunoprecipitation of the proteins and their associated mRNAs from spermatogenic germlines identifies 81 FOG-1 and 722 FOG-3 putative targets. Importantly, almost all FOG-1 targets are also FOG-3 targets, and these common targets are strongly biased for oogenic mRNAs. The discovery of common target mRNAs suggested that FOG-1 and FOG-3 work together. Consistent with that idea, we find that FOG-1 and FOG-3 proteins co-immunoprecipitate from both intact nematodes and mammalian tissue culture cells and that they colocalize in germ cells. Taking our results together, we propose a model in which FOG-1 and FOG-3 work in a complex to repress oogenic transcripts and thereby promote the sperm fate. Experimental Details: WBPaper00048762:Myc-FOG-1_Myc-IP_rep1 WBPaper00048762:Myc-FOG-1_Myc-IP_rep2 WBPaper00048762:Myc-FOG-1_Myc-IP_rep3 WBPaper00048762:Myc-FOG-1_Myc-IP_rep4 WBPaper00048762:Myc-FOG-1_Myc-IP_rep5 WBPaper00048762:Myc-FOG-1_Myc-IP_rep6 WBPaper00048762:Myc-FOG-1_Myc-IP_rep7 WBPaper00048762:N2_Myc-IP_rep1 WBPaper00048762:N2_Myc-IP_rep2 WBPaper00048762:N2_Myc-IP_rep3 WBPaper00048762:N2_Myc-IP_rep4 WBPaper00048762:N2_Myc-IP_rep5 WBPaper00048762:N2_Myc-IP_rep6 WBPaper00048762:N2_Myc-IP_rep7 WBPaper00048762:FOG-3-FLAG_FLAG-IP_rep1 WBPaper00048762:FOG-3-FLAG_FLAG-IP_rep2 WBPaper00048762:FOG-3-FLAG_FLAG-IP_rep3 WBPaper00048762:FOG-3-FLAG_FLAG-IP_rep4 WBPaper00048762:FOG-3-FLAG_FLAG-IP_rep5 WBPaper00048762:FOG-3-FLAG_FLAG-IP_rep6 WBPaper00048762:FOG-3-FLAG_FLAG-IP_rep7 WBPaper00048762:N2_FLAG-IP_rep1 WBPaper00048762:N2_FLAG-IP_rep2 WBPaper00048762:N2_FLAG-IP_rep3 WBPaper00048762:N2_FLAG-IP_rep4 WBPaper00048762:N2_FLAG-IP_rep5 WBPaper00048762:N2_FLAG-IP_rep6 WBPaper00048762:N2_FLAG-IP_rep7 WBPaper00048762:Myc-FOG-1_Myc-WholeAnimal_rep1 WBPaper00048762:Myc-FOG-1_Myc-WholeAnimal_rep2 WBPaper00048762:Myc-FOG-1_Myc-WholeAnimal_rep3 WBPaper00048762:N2_Myc-WholeAnimal_rep1 WBPaper00048762:N2_Myc-WholeAnimal_rep2 WBPaper00048762:N2_Myc-WholeAnimal_rep3 WBPaper00048762:FOG-3-FLAG_FLAG-WholeAnimal_rep1 WBPaper00048762:FOG-3-FLAG_FLAG-WholeAnimal_rep2 WBPaper00048762:FOG-3-FLAG_FLAG-WholeAnimal_rep3 WBPaper00048762:N2_FLAG-WholeAnimal_rep1 WBPaper00048762:N2_FLAG-WholeAnimal_rep2 WBPaper00048762:N2_FLAG-WholeAnimal_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: physical interaction