| Citation | Reinke V, San Gil I, Ward S, Kazmer K. Genome-wide germline-enriched and sex-biased expression profiles in Caenorhabditis elegans. Development, 2004. |
| PubMed ID | 14668411 |
| Short Description | Genome-wide germline-enriched and sex-biased expression profiles in Caenorhabditis elegans. GEO Record: GSE696 GSE715 GSE716 GSE717 GSE718 GSE719 GSE720 GSE721 GSE722 GSE723 GSE724 GSE725 GSE726 GSE727 GSE728 GSE729 GSE730 GSE731 GSE732 GSE733 GSE734 GSE735 GSE736 GSE737 Platform: GPL446 GPL539 GPL540 Download gene-centric, log2 transformed data: WBPaper00006390.ce.mr.csv |
| # of Conditions | 18 |
Full Description
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We performed a genome-wide analysis of gene expression in C. elegans to identify germline- and sex-regulated genes. Using mutants that cause defects in germ cell proliferation or gametogenesis, we identified sets of genes with germline-enriched expression in either hermaphrodites or males, or in both sexes. Additionally, we compared gene expression profiles between males and hermaphrodites lacking germline tissue to define genes with sex-biased expression in terminally differentiated somatic tissues. Cross-referencing hermaphrodite germline and somatic gene sets with in situ hybridization data demonstrates that the vast majority of these genes have appropriate spatial expression patterns. Additionally, we examined gene expression at multiple times during wild-type germline development to define temporal expression profiles for these genes. Sex- and germline-regulated genes have a non-random distribution in the genome, with especially strong biases for and against the X chromosome. Comparison with data from large-scale RNAi screens demonstrates that genes expressed in the oogenic germline display visible phenotypes more frequently than expected. Experimental Details: [cgc6390]:adult_WT_vs_glp-4 [cgc6390]:fem-3(gf)_vs_fem-1(lf) [cgc6390]:glp-4_hermaphrodites_vs_glp-4_him-5_males [cgc6390]:hermaphrodites_vs_him-5_males [cgc6390]:him-5_vs_glp-4_him-5 [cgc6390]:L4_WT_vs_glp-4 [cgc6390]:TP10_27_hr [cgc6390]:TP11_30_hr [cgc6390]:TP12_33_hr [cgc6390]:TP1_0_hr [cgc6390]:TP2_3_hr [cgc6390]:TP3_6_hr [cgc6390]:TP4_9_hr [cgc6390]:TP5_12_hr [cgc6390]:TP6_15_hr [cgc6390]:TP7_18_hr [cgc6390]:TP8_21_hr [cgc6390]:TP9_24_hr. |
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