| Citation | Yee C, Yang W, Hekimi S. The intrinsic apoptosis pathway mediates the pro-longevity response to mitochondrial ROS in C. elegans. Cell, 2014. |
| PubMed ID | 24813612 |
| Short Description | The intrinsic apoptosis pathway mediates the pro-longevity response to mitochondrial ROS in C. elegans. GEO Record: GSE54024 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00045263.ce.mr.csv |
| # of Conditions | 22 |
Full Description
|
The increased longevity of the C. elegans electron transport chain mutants isp-1 and nuo-6 is mediated by mitochondrial ROS (mtROS) signaling. Here we show that the mtROS signal is relayed by the conserved, mitochondria-associated, intrinsic apoptosis signaling pathway (CED-9/Bcl2, CED-4/Apaf1, and CED-3/Casp9) triggered by CED-13, an alternative BH3-only protein. Activation of the pathway by an elevation of mtROS does not affect apoptosis but protects from the consequences of mitochondrial dysfunction by triggering a unique pattern of gene expression that modulates stress sensitivity and promotes survival. In vertebrates, mtROS induce apoptosis through the intrinsic pathway to protect from severely damaged cells. Our observations in nematodes demonstrate that sensing of mtROS by the apoptotic pathway can, independently of apoptosis, elicit protective mechanisms that keep the organism alive under stressful conditions. This results in extended longevity when mtROS generation is inappropriately elevated. These findings clarify the relationships between mitochondria, ROS, apoptosis, and aging. Experimental Details: WBPaper00045263:N2_rep1 WBPaper00045263:N2_rep2 WBPaper00045263:N2_rep3 WBPaper00045263:N2_rep4 WBPaper00045263:isp-1(qm150)_rep1 WBPaper00045263:isp-1(qm150)_rep2 WBPaper00045263:isp-1(qm150)_rep3 WBPaper00045263:nuo-6(qm200)_rep1 WBPaper00045263:nuo-6(qm200)_rep2 WBPaper00045263:nuo-6(qm200)_rep3 WBPaper00045263:0.1mM-paraquat_rep1 WBPaper00045263:0.1mM-paraquat_rep2 WBPaper00045263:0.1mM-paraquat_rep3 WBPaper00045263:ced-4(n1162)_rep1 WBPaper00045263:ced-4(n1162)_rep2 WBPaper00045263:ced-4(n1162)_rep3 WBPaper00045263:isp-1(qm150)ced-4(n1162)_rep1 WBPaper00045263:isp-1(qm150)ced-4(n1162)_rep2 WBPaper00045263:isp-1(qm150)ced-4(n1162)_rep3 WBPaper00045263:nuo-6(qm200);ced-4(n1162)_rep1 WBPaper00045263:nuo-6(qm200);ced-4(n1162)_rep2 WBPaper00045263:nuo-6(qm200);ced-4(n1162)_rep3. |
|
Tags
|
Contact: help (at) wormbase.org
