SPELL - Nematode

Citation	Uno M, Honjoh S, Matsuda M, Hoshikawa H, Kishimoto S, Yamamoto T, Ebisuya M, Matsumoto K, Nishida E. A fasting-responsive signaling pathway that extends life span in C. elegans. Cell Rep, 2013.
PubMed ID	23352664
Short Description	A fasting-responsive signaling pathway that extends life span in C. elegans. GEO Record: GSE27677 GSE42689 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00041960.ce.mr.csv
# of Conditions	56
Full Description	Intermittent fasting is one of the most effective dietary restriction regimens that extend life span in C. elegans and mammals. Fasting-stimulus responses are key to the longevity response; however, the mechanisms that sense and transduce the fasting stimulus remain largely unknown. Through a comprehensive transcriptome analysis in C. elegans, we find that along with the FOXO transcription factor DAF-16, AP-1 (JUN-1/FOS-1) plays a central role in fasting-induced transcriptional changes. KGB-1, one of the C. elegans JNKs, acts as an activator of AP-1 and is activated in response to fasting. KGB-1 and AP-1 are involved in intermittent fasting-induced longevity. Fasting-induced upregulation of the components of the SCF E3 ubiquitin ligase complex via AP-1 and DAF-16 enhances protein ubiquitination and reduces protein carbonylation. Our results thus identify a fasting-responsive KGB-1/AP-1 signaling pathway, which, together with DAF-16, causes transcriptional changes that mediate longevity, partly through regulating proteostasis. Experimental Details: WBPaper00041960:N2_fed_0h_rep1 WBPaper00041960:N2_fed_0h_rep2 WBPaper00041960:N2_fed_24h_rep1 WBPaper00041960:N2_fed_24h_rep2 WBPaper00041960:N2_fed_48h_rep1 WBPaper00041960:N2_fed_48h_rep2 WBPaper00041960:N2_fasted_3h_rep1 WBPaper00041960:N2_fasted_3h_rep2 WBPaper00041960:N2_fasted_6h_rep1 WBPaper00041960:N2_fasted_6h_rep2 WBPaper00041960:N2_fasted_9h_rep1 WBPaper00041960:N2_fasted_9h_rep2 WBPaper00041960:N2_fasted_12h_rep1 WBPaper00041960:N2_fasted_12h_rep2 WBPaper00041960:N2_fasted_18h_rep1 WBPaper00041960:N2_fasted_18h_rep2 WBPaper00041960:N2_fasted_24h_rep1 WBPaper00041960:N2_fasted_24h_rep2 WBPaper00041960:N2_fasted_36h_rep1 WBPaper00041960:N2_fasted_36h_rep2 WBPaper00041960:N2_fasted_48h_rep1 WBPaper00041960:N2_fasted_48h_rep2 WBPaper00041960:N2_fed_rep1 WBPaper00041960:N2_fed_rep2 WBPaper00041960:N2_fed_rep3 WBPaper00041960:N2_fasted_rep1 WBPaper00041960:N2_fasted_rep2 WBPaper00041960:N2_fasted_rep3 WBPaper00041960:kgb-1_fed_rep1 WBPaper00041960:kgb-1_fed_rep2 WBPaper00041960:kgb-1_fed_rep3 WBPaper00041960:kgb-1_fasted_rep1 WBPaper00041960:kgb-1_fasted_rep2 WBPaper00041960:kgb-1_fasted_rep3 WBPaper00041960:jun-1_fed_rep1 WBPaper00041960:jun-1_fed_rep2 WBPaper00041960:jun-1_fed_rep3 WBPaper00041960:jun-1_fasted_rep1 WBPaper00041960:jun-1_fasted_rep2 WBPaper00041960:jun-1_fasted_rep3 WBPaper00041960:daf-16_fed_rep1 WBPaper00041960:daf-16_fed_rep2 WBPaper00041960:daf-16_fed_rep3 WBPaper00041960:daf-16_fasted_rep1 WBPaper00041960:daf-16_fasted_rep2 WBPaper00041960:daf-16_fasted_rep3 WBPaper00041960:mek-1_fed_rep1 WBPaper00041960:mek-1_fed_rep2 WBPaper00041960:mek-1_fed_rep3 WBPaper00041960:mek-1_fasted_rep1 WBPaper00041960:mek-1_fasted_rep2 WBPaper00041960:mek-1_fasted_rep3 WBPaper00041960:mlk-1_fed_rep1 WBPaper00041960:mlk-1_fed_rep2 WBPaper00041960:mlk-1_fasted_rep1 WBPaper00041960:mlk-1_fasted_rep2.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: aging, Topic: response to starvation