SPELL - Nematode

Citation	Leung MC, Rooney JP, Ryde IT, Bernal AJ, Bess AS, Crocker TL, Ji AQ, Meyer JN. Effects of early life exposure to ultraviolet C radiation on mitochondrial DNA content, transcription, ATP production, and oxygen consumption in developing Caenorhabditis elegans. BMC Pharmacol Toxicol, 2013.
PubMed ID	23374645
Short Description	Effects of early life exposure to ultraviolet C radiation on mitochondrial DNA content, transcription, ATP production, and oxygen consumption in developing Caenorhabditis elegans. GEO Record: GSE38997 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00041939.ce.mr.csv
# of Conditions	69
Full Description	BACKGROUND: Mitochondrial DNA (mtDNA) is present in multiple copies per cell and undergoes dramatic amplification during development. The impacts of mtDNA damage incurred early in development are not well understood, especially in the case of types of mtDNA damage that are irreparable, such as ultraviolet C radiation (UVC)-induced photodimers. METHODS: We exposed first larval stage nematodes to UVC using a protocol that results in accumulated mtDNA damage but permits nuclear DNA (nDNA) repair. We then measured the transcriptional response, as well as oxygen consumption, ATP levels, and mtDNA copy number through adulthood. RESULTS: Although the mtDNA damage persisted to the fourth larval stage, we observed only a relatively minor ~40% decrease in mtDNA copy number. Transcriptomic analysis suggested an inhibition of aerobic metabolism and developmental processes; mRNA levels for mtDNA-encoded genes were reduced ~50% at 3 hours post-treatment, but recovered and, in some cases, were upregulated at 24 and 48 hours post-exposure. The mtDNA polymerase was also induced ~8-fold at 48 hours post-exposure. Moreover, ATP levels and oxygen consumption were reduced in response to UVC exposure, with marked reductions of ~50% at the later larval stages. CONCLUSIONS: These results support the hypothesis that early life exposure to mitochondrial genotoxicants could result in mitochondrial dysfunction at later stages of life, thereby highlighting the potential health hazards of time-delayed effects of these genotoxicants in the environment. Experimental Details: WBPaper00041939:control_48h_exp1_1c48 WBPaper00041939:UVC-exposed_48h_exp1_1u48 WBPaper00041939:EtBr-exposed_48h_exp1_1e48 WBPaper00041939:UVC-EtBr-exposed_48h_exp1_1eu48 WBPaper00041939:control_51h_exp1_1c51 WBPaper00041939:UVC-exposed_51h_exp1_1u51 WBPaper00041939:EtBr-exposed_51h_exp1_1e51 WBPaper00041939:UVC-EtBr-exposed_51h_exp1_1eu51 WBPaper00041939:control_51h_exp2_2c51 WBPaper00041939:EtBr-exposed_51h_exp2_2e51 WBPaper00041939:UVC-EtBr-exposed_51h_exp2_2eu51 WBPaper00041939:control_3h_exp4_4c3 WBPaper00041939:UVC-exposed_3h_exp4_4u3 WBPaper00041939:EtBr-exposed_3h_exp4_4e3 WBPaper00041939:UVC-EtBr-exposed_3h_exp4_4eu3 WBPaper00041939:control_24h_exp4_4c24 WBPaper00041939:UVC-exposed_24h_exp4_4u24 WBPaper00041939:EtBr-exposed_24h_exp4_4e24 WBPaper00041939:UVC-EtBr-exposed_24h_exp4_4eu24 WBPaper00041939:control_48h_exp4_4c48 WBPaper00041939:UVC-exposed_48h_exp4_4u48 WBPaper00041939:EtBr-exposed_48h_exp4_4e48 WBPaper00041939:UVC-EtBr-exposed_48h_exp4_4eu48 WBPaper00041939:control_51h_exp4_4c51 WBPaper00041939:UVC-exposed_51h_exp4_4u51 WBPaper00041939:EtBr-exposed_51h_exp4_4e51 WBPaper00041939:UVC-EtBr-exposed_51h_exp4_4eu51 WBPaper00041939:control_3h_exp5_5c3 WBPaper00041939:UVC-exposed_3h_exp5_5u3 WBPaper00041939:EtBr-exposed_3h_exp5_5e3 WBPaper00041939:UVC-EtBr-exposed_3h_exp5_5eu3 WBPaper00041939:control_24h_exp5_5c24 WBPaper00041939:UVC-exposed_24h_exp5_5u24 WBPaper00041939:EtBr-exposed_24h_exp5_5e24 WBPaper00041939:UVC-EtBr-exposed_24h_exp5_5eu24 WBPaper00041939:control_48h_exp5_5c48 WBPaper00041939:UVC-exposed_48h_exp5_5u48 WBPaper00041939:UVC-EtBr-exposed_48h_exp5_5Eu48 WBPaper00041939:control_51h_exp5 WBPaper00041939:UVC-exposed_51h_exp5_5u51 WBPaper00041939:EtBr-exposed_51h_exp5_5e51 WBPaper00041939:UVC-EtBr-exposed_51h_exp5_5Eu51 WBPaper00041939:control_3h_exp6_6c3 WBPaper00041939:UVC-exposed_3h_exp6 WBPaper00041939:EtBr-exposed_3h_exp6 WBPaper00041939:UVC-EtBr-exposed_3h_exp6 WBPaper00041939:control_24h_exp6_6c24 WBPaper00041939:UVC-exposed_24h_exp6_6u24 WBPaper00041939:EtBr-exposed_24h_exp6_6e24 WBPaper00041939:UVC-EtBr-exposed_24h_exp6_6Eu24 WBPaper00041939:EtBr-exposed_48h_exp6_6e48 WBPaper00041939:UVC-EtBr-exposed_48h_exp6_6Eu48 WBPaper00041939:EtBr-exposed_51h_exp6_6e51 WBPaper00041939:UVC-EtBr-exposed_51h_exp6_6Eu51 WBPaper00041939:control_3h_exp7_7c3 WBPaper00041939:UVC-exposed_3h_exp7_7u3 WBPaper00041939:EtBr-exposed_3h_exp7_7e3 WBPaper00041939:UVC-EtBr-exposed_3h_exp7_7eu3 WBPaper00041939:control_24h_exp7_7c24 WBPaper00041939:UVC-exposed_24h_exp7_7u24 WBPaper00041939:EtBr-exposed_24h_exp7_7e24 WBPaper00041939:UVC-EtBr-exposed_24h_exp7_7eu24 WBPaper00041939:control_48h_exp7_7c48 WBPaper00041939:UVC-exposed_48h_exp7_7u48 WBPaper00041939:EtBr-exposed_48h_exp7_7e48 WBPaper00041939:UVC-EtBr-exposed_48h_exp7_7eu48 WBPaper00041939:control_51h_exp7_7c51 WBPaper00041939:UVC-exposed_51h_exp7_7u51 WBPaper00041939:UVC-EtBr-exposed_51h_exp7_7eu51.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: response to chemical, Topic: response to radiation