| Citation | Watson E, MacNeil LT, Arda HE, Zhu LJ, Walhout AJ. Integration of metabolic and gene regulatory networks modulates the C. elegans dietary response. Cell, 2013. |
| PubMed ID | 23540702 |
| Short Description | Integration of metabolic and gene regulatory networks modulates the C. elegans dietary response. GEO Record: GSE43952 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00042204.ce.mr.csv |
| # of Conditions | 9 |
Full Description
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Expression profiles are tailored according to dietary input. However, the networks that control dietary responses remain largely uncharacterized. Here, we combine forward and reverse genetic screens to delineate a network of 184 genes that affect the C. elegans dietary response to Comamonas DA1877 bacteria. We find that perturbation of a mitochondrial network composed of enzymes involved in amino acid metabolism and the TCA cycle affects the dietary response. In humans, mutations in the corresponding genes cause inborn diseases of amino acid metabolism, most of which are treated by dietary intervention. We identify several transcription factors (TFs) that mediate the changes in gene expression upon metabolic network perturbations. Altogether, our findings unveil a transcriptional response system that is poised to sense dietary cues and metabolic imbalances, illustrating extensive communication between metabolic networks in the mitochondria and gene regulatory networks in the nucleus. Experimental Details: WBPaper00042204:N2_fed_DA1877_rep1 WBPaper00042204:N2_fed_DA1877_rep2 WBPaper00042204:N2_fed_DA1877_rep3 WBPaper00042204:metr-1(ok521)_fed_DA1877_rep1 WBPaper00042204:metr-1(ok521)_fed_DA1877_rep2 WBPaper00042204:metr-1(ok521)_fed_DA1877_rep3 WBPaper00042204:pcca-1(ok2282)_fed_DA1877_rep1 WBPaper00042204:pcca-1(ok2282)_fed_DA1877_rep2 WBPaper00042204:pcca-1(ok2282)_fed_DA1877_rep3. |
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